1IHY
GAPDH complexed with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.800, 100.350, 128.310 |
| Unit cell angles | 90.00, 110.28, 90.00 |
Refinement procedure
| Resolution | 24.370 - 3.000 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.19600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.138 | 0.350 |
| Total number of observations | 92755 * | |
| Number of reflections | 35186 | |
| <I/σ(I)> | 7 | 3 |
| Completeness [%] | 97.0 * | 98.5 |
| Redundancy | 6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 298 | Shen, Y.O., (2000) Chin. Sci. Bull., 45, 1199. * |
