1IHX

Crystal structure of two D-glyceraldehyde-3-phosphate dehydrogenase complexes: a case of asymmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	153.409, 100.506, 128.440
Unit cell angles	90.00, 110.49, 90.00

Refinement procedure

Resolution	30.000 * - 2.800
R-factor	0.177
Rwork	0.177
R-free	0.20500 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.410 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.126	0.379 *
Total number of observations	119278 *
Number of reflections	44055
<I/σ(I)>	10	3
Completeness [%]	98.0	97.9
Redundancy	5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	298	Shen, Y.O., (2000) Chin. Sci. Bull., 45, 1199. *