1IHK
CRYSTAL STRUCTURE OF FIBROBLAST GROWTH FACTOR 9 (FGF9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 87.823, 87.823, 115.636 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.081 | 0.264 |
| Total number of observations | 177016 * | |
| Number of reflections | 11766 * | |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | ammonium phosphate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.4 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 25 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | ammonium phosphate | 2 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
