1IHG
Bovine Cyclophilin 40, monoclinic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-06-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.87 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.125, 47.328, 85.563 |
Unit cell angles | 90.00, 119.42, 90.00 |
Refinement procedure
Resolution | 24.000 - 1.800 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CYCLOPHILIN A |
RMSD bond length | 0.070 * |
RMSD bond angle | 0.230 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.096 |
Number of reflections | 41259 | |
<I/σ(I)> | 20.67 | 9.03 |
Completeness [%] | 98.3 | 87.3 |
Redundancy | 3.7 | 1.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 4000, MES Glycerol Sodium Chloride, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 50 (%) | 0.320ml |
2 | 1 | reservoir | MES | 1 (M) | 0.100ml |
3 | 1 | reservoir | glycerol | 50 (%(v/v)) | 0.200ml |
4 | 1 | reservoir | 4 (M) | 0.100 | |
5 | 1 | reservoir | water | 0.280ml | |
6 | 1 | drop | protein | 10-80 (mg/ml) | |
7 | 1 | drop | Tris | 20 (mM) | |
8 | 1 | drop | 100 (mM) |