1IHF
INTEGRATION HOST FACTOR/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03-14 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.360, 59.920, 180.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 * |
| Rwork | 0.192 |
| R-free | 0.25500 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.816) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.063 |
| Number of reflections | 22925 |
| Completeness [%] | 85.2 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | pH 7.40, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | PEG 4000 | ||
| 5 | 1 | 1 | KCL | ||
| 6 | 1 | 1 | SPERMINE | ||
| 7 | 1 | 1 | GLYCEROL | ||
| 8 | 1 | 1 | CDCL2 | ||
| 9 | 1 | 1 | NA AZIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 23 (mg/ml) | |
| 10 | 1 | reservoir | 0.3 (%) | ||
| 11 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | DNA | 1.5 (mM) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 20-23 (%) | |
| 6 | 1 | reservoir | 50 (mM) | ||
| 7 | 1 | reservoir | spermine | 1 (mM) | |
| 8 | 1 | reservoir | glycerol | 5-15 (%) | |
| 9 | 1 | reservoir | 15 (mM) |
