1IH7
High-Resolution Structure of Apo RB69 DNA Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.913 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.196, 116.309, 194.391 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.210 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25800 |
| Structure solution method | phase extension |
| Starting model (for MR) | 1waj with all non-protein atoms removed |
| RMSD bond length | 0.088 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 | 0.737 |
| Number of reflections | 88785 | |
| <I/σ(I)> | 21.9 | 3 |
| Completeness [%] | 98.1 | 98.1 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.25 * | 16 * | Sodium citrate, sodium/potassium tartrate, ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | polymerase | 10-20 (mg/ml) | |
| 2 | 1 | drop | dTTP | 1 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | PEG350 MME | 30 (%(w/v)) | |
| 5 | 1 | reservoir | sodium cacodylate | 100 (mM) |
