1IGY

STRUCTURE OF IMMUNOGLOBULIN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1995-06
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	66.650, 190.660, 73.100
Unit cell angles	90.00, 109.66, 90.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.211
Rwork	0.211
R-free	0.32700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	VL:VH DOMAIN PAIR OF PDB ENTRY 1MCP CL:CH1 DOMAIN PAIR OF PDB ENTRY 2HFL FC FRAGMENT OF PDB ENTRY 1IGT
RMSD bond length	0.009
RMSD bond angle	26.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	3.200
Rmerge	0.145
Number of reflections	27971
<I/σ(I)>	6.4
Completeness [%]	95.6
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.1		Harris, L.J., (1995) Proteins: Struct.,Funct., Genet., 23, 285. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	12 (%(w/v))