1IGR

Type 1 Insulin-like growth factor receptor (DOMAINS 1-3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1996-11-15
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	77.390, 99.720, 120.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.600
R-factor	0.237 *
Rwork	0.237
R-free	0.30400
Structure solution method	MIRAS
RMSD bond length	0.017
RMSD bond angle	0.048
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.064	0.519
Number of reflections	26963
<I/σ(I)>	18.7	3.3
Completeness [%]	99.6	97.4
Redundancy	4.1	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		Mckern, N.M., (1997) Protein Sci., 6, 2663. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	drop	Tris-HCl	10-20 (mM)
3	1	drop	azide	0.02 (%(w/v))