1IGQ
C-terminal Domain of Transcriptional Repressor Protein KorB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0721 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.130, 82.150, 87.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.000 - 1.700 |
R-factor | 0.1946 |
Rwork | 0.193 |
R-free | 0.22800 |
Structure solution method | MIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 2.098 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.044 | 0.203 |
Number of reflections | 32160 | |
<I/σ(I)> | 10.4 | 3.8 |
Completeness [%] | 98.9 | 95.7 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 293 | PEG4000, NaCl, Na acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.6 |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | reservoir | PEG4000 | 36 (%) | |
4 | 1 | reservoir | 200 (mM) | ||
5 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |