1IGN
DNA-BINDING DOMAIN OF RAP1 IN COMPLEX WITH TELOMERIC DNA SITE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1995-02-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 |
| Unit cell lengths | 90.610, 90.610, 80.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.000 - 2.250 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.29400 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.900 |
| Data reduction software | MOSFLM |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 16.000 |
| High resolution limit [Å] | 2.250 |
| Rmerge | 0.047 |
| Number of reflections | 33281 |
| Completeness [%] | 96.0 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-DNA complex | 0.08-0.1 (mM) | |
| 2 | 1 | drop | MES | 20 (mM) | pH6.0 |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | spermine | 2 (mM) | |
| 5 | 1 | drop | MPD | 10-20 (%) | |
| 6 | 1 | reservoir | MPD | 40 (%) |
