1IGD

THE THIRD IGG-BINDING DOMAIN FROM STREPTOCOCCAL PROTEIN G: AN ANALYSIS BY X-RAY CRYSTALLOGRAPHY OF THE STRUCTURE ALONE AND IN A COMPLEX WITH FAB

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	34.900, 40.300, 42.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.100
R-factor	0.193
RMSD bond length	0.011
RMSD bond angle	0.032
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	1.100 *
Rmerge	0.058 *
Number of reflections	23530
Completeness [%]	95.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	reservoir solution		0.01 ml
3	1	reservoir	sodium acetate	10 (mM)
4	1	reservoir	sodium azide	0.01 (%(w/v))
5	1	reservoir	PEG4000	24-26 (%(w/v))