1IGB

AEROMONAS PROTEOLYTICA AMINOPEPTIDASE COMPLEXED WITH THE INHIBITOR PARA-IODO-D-PHENYLALANINE HYDROXAMATE

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	291
Detector technology	AREA DETECTOR
Collection date	1993-09
Detector	XENTRONICS
Spacegroup name	P 61 2 2
Unit cell lengths	109.150, 109.150, 98.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.16
Rwork	0.160
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT SOFTWARE USED : X-PLOR STARTING MODEL FOR MOLECULAR REPLACEMENT: NATIVE PROTEIN (REFERENCE 1)
RMSD bond length	0.015
RMSD bond angle	24.800 *
Data reduction software	CCP4 (ROTAVATA-AGROVATA)
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.081
Total number of observations	118144 *
Number of reflections	15274
Completeness [%]	91.7
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	AAP	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop	KSCN	10 (mM)
4	1	drop		0.4 (M)
5	1	reservoir	HEPES	110 (mM)
6	1	reservoir		4.5 (M)