1IFV
CRYSTAL STRUCTURE OF PATHOGENESIS-RELATED PROTEIN LLPR10.1B FROM YELLOW LUPINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.880, 107.445, 46.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.250 |
| R-factor | 0.186 * |
| Rwork | 0.186 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LlPR10.1A |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.102 | 0.399 |
| Total number of observations | 88882 * | |
| Number of reflections | 15594 * | |
| <I/σ(I)> | 13.26 | 2.78 |
| Completeness [%] | 97.2 | 94.7 |
| Redundancy | 5.6 * | 3.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 * | 292 | sodium citrate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5.6 (mg/ml) | |
| 2 | 1 | 1 | sodium citrate | 0.9 (M) | |
| 3 | 1 | 1 | HEPES | 0.1 (M) | pH8.0 |
