1IFU

RICIN A-CHAIN (RECOMBINANT) COMPLEX WITH FORMYCIN

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	68.700, 68.700, 141.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 * - 2.400
R-factor	0.186 *
Rwork	0.212
RMSD bond length	0.015 *
RMSD bond angle	2.800 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.000 *
High resolution limit [Å]	2.400 *	2.380 *
Rmerge	0.065 *	0.227 *
Number of reflections	12376 *
Completeness [%]	92.9 *	55.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.9 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.875 (mg/ml)
2	1	drop	Tris-HCl	56.2 (mM)
3	1	drop	EDTA	0.75 (mM)
4	1	drop	dithiothreitol	3.75 (mM)
5	1	drop	sodium citrate	16.7 (mM)
6	1	drop	ammonium sulfate	10 (%(v/v))
7	1	reservoir	sodium citrate	50 (mM)
8	1	reservoir	ammonium sulfate	30 (%(v/v))