1IFR
Structure of Lamin A/C Globular Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.430, 52.550, 67.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.400 |
| Rwork | 0.216 |
| R-free | 0.23800 * |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.410 * |
| Refinement software | CNS |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG4000, ammonium acetate, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%) | |
| 2 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 5 | 1 | drop | protein | 20 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 7 | 1 | drop | 0.1 (M) | ||
| 8 | 1 | drop | dithiothreitol | 10 (mM) |
