1IEJ
OVOTRANSFERRIN, N-TERMINAL LOBE, HOLO FORM, AT 1.65 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-09-12 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.460, 85.950, 76.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.650 |
| R-factor | 0.173 * |
| Rwork | 0.173 |
| R-free | 0.24200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.200 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.620 |
| Rmerge | 0.058 |
| Total number of observations | 135942 * |
| Number of reflections | 35322 |
| Completeness [%] | 89.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | used to seed, Dewan, J.C., (1993) Biochemistry, 32, 11963. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 59 (mg/ml) | |
| 2 | 1 | drop | acetate | 0.02 (M) | |
| 3 | 1 | drop | 0.01 (%) | ||
| 4 | 1 | drop | PEG6000 | 4-5 (%) | |
| 5 | 1 | drop | acetate | 0.02 (M) | |
| 6 | 1 | reservoir | PEG6000 | 4-5 (%) | |
| 7 | 1 | reservoir | acetate | 0.02 (M) |
