1IDW
STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[CL]U IN PRESENCE OF RH(NH3)6+++
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1997-03-20 |
| Detector | ENRAF-NONIUS FAST |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.820, 19.380, 50.300 |
| Unit cell angles | 90.00, 109.90, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.20200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 165d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.032 |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 2.000 |
| High resolution limit [Å] | 1.800 * | 1.800 |
| Rmerge | 0.039 | 0.212 |
| Total number of observations | 13027 * | |
| Number of reflections | 3641 | |
| <I/σ(I)> | 18 | 3 |
| Completeness [%] | 85.0 * | 43.2 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | 277 | cacodylate buffer, MPD, Rhodium-hexammine, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate buffer | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | rhodium-hexammine | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | lithium cacodylate | 0.05 (M) | |
| 2 | 1 | drop | RNA/DNA hybrid | 0.1mg | |
| 3 | 1 | drop | rhodium/iridium hexammine | 0.1mg | |
| 4 | 1 | drop | 5 (%) | ||
| 5 | 1 | reservoir | MPD | 35-40 (%) |
