1IDK
PECTIN LYASE A
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.400, 83.500, 93.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.930 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.23200 |
| Structure solution method | MR + MIR |
| Starting model (for MR) | DERIVED FROM BACILLUS SUBTILIS PECTATE LYASE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 (V. 2.13 (AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.440 | 1.970 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.061 | 0.250 |
| Total number of observations | 80561 * | |
| Number of reflections | 25695 | |
| <I/σ(I)> | 19.47 | 5.3 |
| Completeness [%] | 94.0 | 84.3 * |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | Jenkins, J., (1996) Acta Crystallogr.,Sect.D, 52, 402. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20.4 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 9.3 (%) | |
| 3 | 1 | drop | sodium acetate | 33 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 28 (%) | |
| 5 | 1 | reservoir | sodium acetate | 100 (mM) |
