1IDE

ISOCITRATE DEHYDROGENASE Y160F MUTANT STEADY-STATE INTERMEDIATE COMPLEX (LAUE DETERMINATION)

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Detector technology	IMAGE PLATE
Collection date	1993-10-15
Detector	FUJI
Wavelength(s)	0.7 - 2.1
Spacegroup name	P 43 21 2
Unit cell lengths	105.100, 105.100, 150.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.500
R-factor	0.192
Rwork	0.192
RMSD bond length	0.019
RMSD bond angle	26.200 *
Data reduction software	OXFORD (LAUE PACKAGE (J.CAMPBELL))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.084
Number of reflections	17043
Completeness [%]	61.0
Redundancy	17.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	dephosphorylated IDH	28 (mg/ml)
2	1	reservoir	ammonium sulfate	34 (%sat)
3	1	reservoir		100 (mM)
4	1	reservoir		35 (mM)
5	1	reservoir	citric acid	9 (mM)
6	1	reservoir	dithiothreitol	0.2 (mM)