1ID9
STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[F]U IN PRESENCE OF RH(NH3)6+++
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.967 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.730, 19.390, 45.830 |
| Unit cell angles | 90.00, 105.70, 90.00 |
Refinement procedure
| Resolution | 11.000 - 1.600 |
| R-factor | 0.153 |
| Rwork | 0.156 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 165d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.027 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 * | 0.141 |
| Total number of observations | 38535 * | |
| Number of reflections | 4339 | |
| <I/σ(I)> | 21 | 4.1 |
| Completeness [%] | 92.0 * | 76 |
| Redundancy | 9.0 * | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | 277 | Cacodylate, MPD, rhodium(iii) hexammine, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Cacodylate | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | rhodium(iii) hexammine | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | lithium cacodylate | 0.05 (M) | |
| 2 | 1 | drop | RNA/DNA hybrid | 0.1mg | |
| 3 | 1 | drop | rhodium/iridium hexammine | 0.1mg | |
| 4 | 1 | drop | 5 (%) | ||
| 5 | 1 | reservoir | MPD | 35-40 (%) |
