1ID9
STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[F]U IN PRESENCE OF RH(NH3)6+++
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.967 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 53.730, 19.390, 45.830 |
Unit cell angles | 90.00, 105.70, 90.00 |
Refinement procedure
Resolution | 11.000 - 1.600 |
R-factor | 0.153 |
Rwork | 0.156 |
R-free | 0.19500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 165d |
RMSD bond length | 0.013 |
RMSD bond angle | 0.027 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.057 * | 0.141 |
Total number of observations | 38535 * | |
Number of reflections | 4339 | |
<I/σ(I)> | 21 | 4.1 |
Completeness [%] | 92.0 * | 76 |
Redundancy | 9.0 * | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | 277 | Cacodylate, MPD, rhodium(iii) hexammine, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Cacodylate | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | rhodium(iii) hexammine | ||
4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | lithium cacodylate | 0.05 (M) | |
2 | 1 | drop | RNA/DNA hybrid | 0.1mg | |
3 | 1 | drop | rhodium/iridium hexammine | 0.1mg | |
4 | 1 | drop | 5 (%) | ||
5 | 1 | reservoir | MPD | 35-40 (%) |