1ID1

CRYSTAL STRUCTURE OF THE RCK DOMAIN FROM E.COLI POTASSIUM CHANNEL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-01
Detector	BRANDEIS
Wavelength(s)	1.1
Spacegroup name	P 41
Unit cell lengths	65.510, 65.510, 110.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.208
Rwork	0.208
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	30.000
High resolution limit [Å]	2.400 *	2.400
Rmerge	0.086 *	0.292 *
Completeness [%]	99.5 *	96.3 *
Redundancy	4.8 *	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	20 *	PEG 4000, sodium chloride, tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		10 (mM)
4	1	reservoir	PEG4000	10 (%)
5	1	reservoir		1-1.5 (M)
6	1	reservoir	Tris-HCl	100 (mM)