1ICX

CRYSTAL STRUCTURE OF PATHOGENESIS-RELATED PROTEIN LLPR10.1A FROM YELLOW LUPINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-07-17
Detector	MARRESEARCH
Wavelength(s)	1.3750
Spacegroup name	P 21 21 21
Unit cell lengths	34.973, 56.617, 61.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.950
R-factor	0.196
Rwork	0.196
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1btv
RMSD bond length	0.016
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.053	0.127
Total number of observations	89375 *
Number of reflections	9446
<I/σ(I)>	25.73	8.46
Completeness [%]	99.5	96.8
Redundancy	9.5 *	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	ammonium sulfate, TRIS, sucrose laureate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K