1ICG
STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[F]U IN PRESENCE OF IR(NH3)6+++
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.967 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 53.750, 19.400, 45.840 |
Unit cell angles | 90.00, 105.70, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.530 |
R-factor | 0.177 |
Rwork | 0.177 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 165d |
RMSD bond length | 0.012 |
RMSD bond angle | 0.026 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.600 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.044 | 0.156 |
Total number of observations | 42723 * | |
Number of reflections | 6518 | |
<I/σ(I)> | 25 | 4.4 |
Completeness [%] | 91.5 | 75 |
Redundancy | 6.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | cacodylate, iridium hexammine, MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | cacodylate | ||
2 | 1 | 1 | Iridium hexammine | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | lithium cacodylate | 0.05 (M) | |
2 | 1 | drop | RNA/DNA hybrid | 0.1mg | |
3 | 1 | drop | rhodium/iridium hexammine | 0.1mg | |
4 | 1 | drop | 5 (%) | ||
5 | 1 | reservoir | MPD | 35-40 (%) |