1IC8
HEPATOCYTE NUCLEAR FACTOR 1A BOUND TO DNA : MODY3 GENE PRODUCT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-08-04 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.0673 |
| Spacegroup name | P 41 |
| Unit cell lengths | 50.389, 50.389, 207.272 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.600 |
| R-factor | 0.26 |
| Rwork | 0.254 |
| R-free | 0.31200 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 * |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.052 |
| Number of reflections | 14836 |
| <I/σ(I)> | 14.35 |
| Completeness [%] | 93.4 |
| Redundancy | 3.23 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 50mM Imidazole, 32% PEG8K, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Imidazole | ||
| 2 | 1 | 1 | PEG8K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-30 (mg/ml) | |
| 2 | 1 | reservoir | imidazole | 100 (mM) | pH8.5 |
| 3 | 1 | reservoir | PEG8000 | 30-33 (%) |
