1IC1
THE CRYSTAL STRUCTURE FOR THE N-TERMINAL TWO DOMAINS OF ICAM-1
Experimental procedure
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 88.000, 42.200, 93.000 |
| Unit cell angles | 90.00, 109.30, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.27900 |
| Structure solution method | SINGLE ISOMORPHOUS REPLACEMENT AND MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1ZXQ AND 1VSC |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.808 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.101 | 0.389 |
| Number of reflections | 13176 | |
| <I/σ(I)> | 6.2 | 2 |
| Completeness [%] | 95.0 | 97.4 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | 17% PEG 4000, NA CACODYLATE, PH 6.5, AND 100 MM B-OCTYL-GLUCOPYRANOSIDE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | PEG4000 | 8.5 (%) | |
| 4 | 1 | drop | beta-octyl glucopyranoside | 50 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 17 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 7 | 1 | reservoir | beta-octyl glucopyranoside | 100 (mM) |
