1IBS
PHOSPHORIBOSYLDIPHOSPHATE SYNTHETASE IN COMPLEX WITH CADMIUM IONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 274 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 115.600, 115.600, 107.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.948 * |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.136 | 0.400 |
| Number of reflections | 19806 | |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 98.0 | 96.2 |
| Redundancy | 4.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Bentsen, A.K., (1996) Proteins 24. 238. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.7 (mg/ml) | |
| 10 | 1 | reservoir | PEG400 | 2.0 (%(v/v)) | |
| 2 | 1 | drop | mATP | 1.8 (mM) | |
| 3 | 1 | drop | ADP | 2 (mM) | |
| 4 | 1 | drop | Rib-5-P | 4.4 (mM) | |
| 5 | 1 | drop | 9.8 (mM) | ||
| 6 | 1 | drop | NaPi | 50 (mM) | pH7.5 |
| 7 | 1 | drop | beta-octylglucoside | 0.5 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.5 |
| 9 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
