1IBR
COMPLEX OF RAN WITH IMPORTIN BETA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.700, 108.950, 114.050 |
| Unit cell angles | 90.00, 100.66, 90.00 |
Refinement procedure
| Resolution | 28.700 - 2.300 |
| R-factor | 0.246 * |
| Rwork | 0.245 |
| R-free | 0.27200 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.036 | 0.011 |
| Total number of observations | 354933 * | |
| Number of reflections | 69758 | |
| <I/σ(I)> | 28.1 | 8 |
| Completeness [%] | 96.6 | 93.8 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 9.5 (%) | |
| 2 | 1 | 1 | calcium acetate | 110 (mM) | |
| 3 | 1 | 1 | MES | 100 (mM) | |
| 4 | 1 | 1 | dioxane | 0.5 (%) |
