1IBM
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH A MESSENGER RNA FRAGMENT AND COGNATE TRANSFER RNA ANTICODON STEM-LOOP BOUND AT THE A SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-02-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.99187 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 401.574, 401.574, 176.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 316.230 - 3.310 |
| R-factor | 0.231 * |
| Rwork | 0.231 |
| R-free | 0.28600 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 28.350 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 316.230 | 3.430 |
| High resolution limit [Å] | 3.310 | 3.310 |
| Rmerge | 0.122 * | 0.654 * |
| Total number of observations | 968646 * | |
| Number of reflections | 206478 | |
| <I/σ(I)> | 10.9 | 2.6 |
| Completeness [%] | 97.5 | 93.6 |
| Redundancy | 4.69 | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | Wimberly, B.T., (2000) Nature, 407, 327.,Trakhanov, S.D., (1987) FEBS Lett., 220, 319. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4Cl | ||
| 10 | 1 | 2 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | sodium cacodylate | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | NH4Cl | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | magnesium acetate | ||
| 9 | 1 | 2 | sodium cacodylate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 250 (mM) | ||
| 10 | 1 | drop | 75 (mM) | ||
| 11 | 1 | drop | 200 (mM) | ||
| 12 | 1 | drop | MPD | 10 (%) | |
| 2 | 1 | reservoir | 75 (mM) | ||
| 3 | 1 | reservoir | 25 (mM) | ||
| 4 | 1 | reservoir | 2-mercaptoethanol | 6 (mM) | |
| 5 | 1 | reservoir | potassium cacodylate | 0.1 (M) | or 0.1M MES |
| 6 | 1 | reservoir | MPD | 13-17 (%) | |
| 7 | 1 | drop | protein | 10 (mg/ml) | |
| 8 | 1 | drop | Tris-HCl | 20 (mM) | |
| 9 | 1 | drop | 25 (mM) |
