1IB2
CRYSTAL STRUCTURE OF A PUMILIO-HOMOLOGY DOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2000-12-22 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 142.880, 142.880, 111.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 71.440 - 1.900 |
R-factor | 0.1955 * |
Rwork | 0.198 |
R-free | 0.24200 |
Structure solution method | MIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 19.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.400 | 1.920 |
High resolution limit [Å] | 1.900 * | 1.900 * |
Rmerge | 0.063 * | 0.326 * |
Total number of observations | 189138 * | |
Number of reflections | 37094 * | 3405 * |
<I/σ(I)> | 21.2 | 2.3 |
Completeness [%] | 99.6 | 99.2 * |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 4 * | PEG 6000, Lithium sulphate, sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5.5 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 12 (%(w/v)) | |
3 | 1 | reservoir | 100 (mM) | ||
4 | 1 | reservoir | sodium citrate | 100 (mM) |