1I9V
CRYSTAL STRUCTURE ANALYSIS OF A TRNA-NEOMYCIN COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.104 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.900, 32.900, 63.100 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.2297 |
| Rwork | 0.228 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tna |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.600 | 2.600 * |
| Rmerge | 0.050 | 0.195 * |
| Number of reflections | 6515 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 90.6 | 76 * |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 6 * | MgCl, MPD, Cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 281K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | Cacodylate | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 8 (%) | |
| 2 | 1 | reservoir | sodium cacodylate | 80 (mM) | |
| 3 | 1 | reservoir | 40 (mM) | ||
| 4 | 1 | drop | protein | 25 (mg/ml) | |
| 5 | 1 | drop | 2 (mM) | ||
| 6 | 1 | drop | neomycin B | 10 (mM) |
