1I9T
CRYSTAL STRUCTURE OF THE OXIDIZED RNA TRIPHOSPHATASE DOMAIN OF MOUSE MRNA CAPPING ENZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-08-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.20374 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.002, 98.961, 71.627 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.000 - 1.700 |
R-factor | 0.193 |
Rwork | 0.202 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i9s |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.700 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (BODY)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.155 |
Total number of observations | 145070 * | |
Number of reflections | 24075 | |
<I/σ(I)> | 7.8 | 4.9 |
Completeness [%] | 98.0 | 96.5 |
Redundancy | 6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 10 * | 15% isopropanol, 50mM sodium cacodylate (pH 6.5), 25mM magnesium chloride, 25mM ammonium sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | isopropanol | 15 (%) | |
3 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
4 | 1 | reservoir | 25 (mM) | ||
5 | 1 | reservoir | ammonium sulfate | 25 (mM) |