1I9H

CORE STREPTAVIDIN-BNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-06-20
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54
Spacegroup name	I 2 2 2
Unit cell lengths	47.121, 95.160, 105.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.177 *
Rwork	0.177
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2izf
RMSD bond length	0.006 *
RMSD bond angle	1.290 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.098	0.265
Number of reflections	8647
<I/σ(I)>	10.9
Completeness [%]	90.7	94.2
Redundancy	2. *	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% isopropanol, 0.1M NaCitrate, 0.05M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.2 (M)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir	isopropanol	20 (%)