1I9A
STRUCTURAL STUDIES OF CHOLESTEROL BIOSYNTHESIS: MEVALONATE 5-DIPHOSPHATE DECARBOXYLASE AND ISOPENTENYL DIPHOSPHATE ISOMERASE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2000-12-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97940, 0.97900 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 72.758, 72.758, 204.471 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.390 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.308 |
| Number of reflections | 34202 | |
| <I/σ(I)> | 18.2 | 3.5 |
| Completeness [%] | 96.2 | 91.1 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 8% PEG 8K, 15% PEG 1K 100mM NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
