1I97
CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH TETRACYCLINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2000-09-01 |
| Detector | SBC |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 406.900, 406.900, 175.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 4.500 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.25400 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 4.580 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.099 | 0.310 |
| Number of reflections | 79036 | |
| <I/σ(I)> | 9.1 | 3.4 |
| Completeness [%] | 89.7 | 79.8 |
| Redundancy | 4.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | spermidine | ||
| 4 | 1 | 2 | MPD |
