1I96
CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH THE TRANSLATION INITIATION FACTOR IF3 (C-TERMINAL DOMAIN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2000-12-01 |
| Detector | SBC |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 407.500, 407.500, 174.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 4.200 |
| R-factor | 0.211 |
| Rwork | 0.205 |
| R-free | 0.26400 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 4.270 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.099 | 0.357 |
| Number of reflections | 83090 | |
| <I/σ(I)> | 9.2 | 2.4 |
| Completeness [%] | 81.5 | 69.6 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | spermidine | ||
| 4 | 1 | 2 | MPD |
