1I94
CRYSTAL STRUCTURES OF THE SMALL RIBOSOMAL SUBUNIT WITH TETRACYCLINE, EDEINE AND IF3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2000-12-01 |
| Detector | SBC |
| Wavelength(s) | 1.2096 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 406.300, 406.300, 173.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 3.200 |
| R-factor | 0.207 |
| Rwork | 0.203 |
| R-free | 0.24500 |
| Structure solution method | MIR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.014 | 0.381 |
| Number of reflections | 221133 | |
| <I/σ(I)> | 19.8 | 2 |
| Completeness [%] | 86.8 | 83.2 |
| Redundancy | 11 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | magnesium chloride | ||
| 3 | 1 | 1 | spermidine |
