1I94
CRYSTAL STRUCTURES OF THE SMALL RIBOSOMAL SUBUNIT WITH TETRACYCLINE, EDEINE AND IF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2000-12-01 |
Detector | SBC |
Wavelength(s) | 1.2096 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 406.300, 406.300, 173.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 3.200 |
R-factor | 0.207 |
Rwork | 0.203 |
R-free | 0.24500 |
Structure solution method | MIR |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.014 | 0.381 |
Number of reflections | 221133 | |
<I/σ(I)> | 19.8 | 2 |
Completeness [%] | 86.8 | 83.2 |
Redundancy | 11 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | or 19 degrees centigrade * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | magnesium chloride | ||
3 | 1 | 1 | spermidine |