1I92
STRUCTURAL BASIS OF THE NHERF PDZ1-CFTR INTERACTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2000-11-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 51.658, 51.658, 66.966 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.810 - 1.700 |
R-factor | 0.187 * |
Rwork | 0.187 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g9o |
RMSD bond length | 0.018 |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.810 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.053 | 0.300 |
Total number of observations | 110148 * | |
Number of reflections | 10950 | |
<I/σ(I)> | 27.6 | 4 |
Completeness [%] | 92.6 | 40 * |
Redundancy | 9.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 20 * | Sodium Acetate, Sodium Chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
3 | 1 | reservoir | 2 (M) |