1I8T
STRUCTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2000-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.642, 98.123, 132.476 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.185 * |
| Rwork | 0.185 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.510 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | COMPLEX (PROBLEM DISCUSSED IN REFERENCE) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.520 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.059 | 0.224 |
| Number of reflections | 28288 | |
| <I/σ(I)> | 10.9 | 3.3 |
| Completeness [%] | 98.0 | 93 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 298 | used macroseeding, Sanders, D.A.R., (2001) Acta Crystallogr., Sect.D, 57, 1415. * |
| 1 | Vapor diffusion * | 7 * | 298 | used macroseeding, Sanders, D.A.R., (2001) Acta Crystallogr., Sect.D, 57, 1415. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG6000 | 10-20 (%) | |
| 2 | 1 | drop | HEPES | 100 (mM) | |
| 3 | 1 | drop | L-cysteine | 0.01 (M) | |
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | protein | 2-3 (mg/ml) |
