1I8T

STRUCTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	130
Detector technology	CCD
Collection date	2000-02-28
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 21 21 21
Unit cell lengths	56.642, 98.123, 132.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.400
R-factor	0.185 *
Rwork	0.185
R-free	0.24500
Structure solution method	MAD
RMSD bond length	0.012 *
RMSD bond angle	1.510 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	COMPLEX (PROBLEM DISCUSSED IN REFERENCE)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.520
High resolution limit [Å]	2.400	2.400
Rmerge	0.059	0.224
Number of reflections	28288
<I/σ(I)>	10.9	3.3
Completeness [%]	98.0	93
Redundancy	3.9	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	298	used macroseeding, Sanders, D.A.R., (2001) Acta Crystallogr., Sect.D, 57, 1415. *
1	Vapor diffusion *	7 *	298	used macroseeding, Sanders, D.A.R., (2001) Acta Crystallogr., Sect.D, 57, 1415. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	10-20 (%)
2	1	drop	HEPES	100 (mM)
3	1	drop	L-cysteine	0.01 (M)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop	protein	2-3 (mg/ml)