1I8Q
CRYSTAL STRUCTURE OF STREPTOCOCCUS AGALACTIAE HYALURONATE LYASE COMPLEXED WITH ENZYME PRODUCT, UNSATURATED DISACCHARIDE HYALURONAN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-25 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.030, 155.017, 237.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.182 * |
| Rwork | 0.182 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | native structure of streptococcus agalactiae hyaluronate lyase |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.152 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.200 | |
| Rmerge | 0.123 | 0.216 * |
| Total number of observations | 295197 * | |
| Number of reflections | 45510 * | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 98.1 | 91.5 * |
| Redundancy | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | Jedrzejas, M.J., (2000) Acta Crystallogr., Sect.D, 56, 460. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | cacodylate | 100 (mM) | pH6.0 |
| 2 | 1 | reservoir | KSCN | 30 (mM) | |
| 3 | 1 | reservoir | PEG5000 MME | 19 (%) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | HEPES | 10 (mM) | |
| 6 | 1 | drop | EDTA | 2 (mM) | pH8.0 |
