1I8O
RHODOPSEUDOMONAS PALUSTRIS CYT C2 AMMONIA COMPLEX AT 1.15 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.950, 64.950, 68.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 1.150 |
| R-factor | 0.139 * |
| Rwork | 0.135 |
| R-free | 0.16700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hh7 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | SHELX (VERSION 97-1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.200 | 1.210 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.054 | 0.412 |
| Total number of observations | 364867 * | |
| Number of reflections | 57362 * | |
| <I/σ(I)> | 7.1 | 5.9 |
| Completeness [%] | 99.3 * | 96.7 * |
| Redundancy | 6.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 293 | 60% AMMONIUM SULPHATE, 0.1 M TRIS PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.0 (mg/ml) | pH6.0 |
| 2 | 1 | drop | phosphate | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 42-44 (%sat) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) | pH4.4 |
