1I8N
CRYSTAL STRUCTURE OF LEECH ANTI-PLATELET PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8825 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 73.450, 73.450, 199.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.215 * |
| Rwork | 0.215 |
| R-free | 0.24000 |
| Structure solution method | SIR |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.037 | 0.253 |
| Number of reflections | 27864 | |
| <I/σ(I)> | 35.6 | 5.1 |
| Completeness [%] | 97.1 | 96.5 |
| Redundancy | 4.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 277 | MPD, sodium citrate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 10 (mM) | |
| 3 | 1 | reservoir | 2-propanol | 20 (%(v/v)) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) |
