1I89
Chalcone synthase (G256L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.103, 98.103, 131.132 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.200 - 1.860 |
| R-factor | 0.206 * |
| Rwork | 0.215 |
| R-free | 0.25900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | wild-type CHS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.200 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.042 | 0.145 |
| Total number of observations | 390574 * | |
| Number of reflections | 57245 | |
| <I/σ(I)> | 21.9 | 5.7 |
| Completeness [%] | 97.3 | 85 |
| Redundancy | 6.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | 2.2-2.4 M ammonium sulfate, 0.1 M PIPES, 5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.2-2.4 (M) | |
| 3 | 1 | reservoir | PIPES | 0.1 (M) | pH6.5 |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) |
