1I86
CHALCONE SYNTHASE, G256A MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2000-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 98.096, 98.096, 65.066 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.200 - 1.500 |
| R-factor | 0.185 * |
| Rwork | 0.187 |
| R-free | 0.22100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | wild-type CHS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.039 | 0.373 |
| Total number of observations | 383046 * | |
| Number of reflections | 53301 | |
| <I/σ(I)> | 29.7 | 2.4 |
| Completeness [%] | 91.4 | 57.3 |
| Redundancy | 7.19 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | 2.2-2.4 M ammonium sulfate, 100 mM PIPES, 5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.2-2.4 (M) | |
| 3 | 1 | reservoir | PIPES | 0.1 (M) | pH6.5 |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) |
