1I81

CRYSTAL STRUCTURE OF A HEPTAMERIC LSM PROTEIN FROM METHANOBACTERIUM THERMOAUTOTROPHICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-07-01
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	40.380, 71.940, 94.670
Unit cell angles	90.00, 93.71, 90.00

Refinement procedure

Resolution	15.000 - 2.000
Rwork	0.218
R-free	0.25700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Model constructed from 1B34
RMSD bond length	0.005 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.000	2.000 *
Rmerge	0.049	0.307 *
Total number of observations	119267 *
Number of reflections	35595 *
<I/σ(I)>	20.7
Completeness [%]	98.2 *	85.6 *
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	21 *	Tris PEG3350 Lithium sulphate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)
2	1	reservoir	PEG3350	16 (%(v/v))
3	1	reservoir	lithium sulfate	0.25 (M)