1I7U
CRYSTAL STRUCTURE OF CLASS I MHC A2 IN COMPLEX WITH PEPTIDE P1049-6V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0789 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.461, 63.333, 75.436 |
| Unit cell angles | 81.94, 76.44, 77.94 |
Refinement procedure
| Resolution | 50.000 * - 1.850* |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.880 |
| High resolution limit [Å] | 1.850 * | 1.850 |
| Rmerge | 0.040 | 0.178 |
| Number of reflections | 73305 | |
| <I/σ(I)> | 9.3 | 2.7 |
| Completeness [%] | 97.2 | 95.5 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 14 (%) | |
| 2 | 1 | reservoir | MES | 25 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | MES | 25 (mM) |
