1I7P
CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2000-10-11 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.083, 70.666, 78.986 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.215 * |
| Rwork | 0.215 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PROTEIN ATOMS OF 1NDH |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.216 |
| Number of reflections | 26290 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 6.2 * | 5.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 6000, MPD, SODIUM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 8 (%) | |
| 2 | 1 | reservoir | MPD | 5 (%) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
