1I7H
CRYSTAL STURCUTURE OF FDX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 140 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.501, 107.501, 85.682 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 1.700 |
| R-factor | 0.255 * |
| Rwork | 0.262 |
| R-free | 0.27800 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.066 | 0.273 |
| Total number of observations | 155486 * | |
| Number of reflections | 40083 * | |
| <I/σ(I)> | 6.4 | 1.2 |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 3.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 23 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG4000 | 33 (%) | |
| 4 | 1 | reservoir | 2-butanol | 5 (%) | |
| 5 | 1 | reservoir | 80 (mM) |
