1I71
HIGH RESOLUTION CRYSTAL STRUCTURE OF APOLIPOPROTEIN(A) KRINGLE IV TYPE 7: INSIGHTS INTO LIGAND BINDING
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9479 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.110, 40.490, 65.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.450 |
| R-factor | 0.171 * |
| Rwork | 0.173 |
| R-free | 0.19600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pk4 |
| RMSD bond length | 0.044 * |
| RMSD bond angle | 1.420 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.069 * | 0.200 |
| Total number of observations | 48859 * | |
| Number of reflections | 15774 * | |
| <I/σ(I)> | 14.8 | 2.6 |
| Completeness [%] | 91.8 * | 96.7 |
| Redundancy | 2.9 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Ammonium sulfate, 2-[N-morpholino]ethane sulfonic acid, , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 21-23 (%) | |
| 2 | 1 | reservoir | MES | 0.1 (M) |
