1I6X

STRUCTURE OF A STAR MUTANT CRP-CAMP AT 2.2 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1998-08-21
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.262, 93.343, 105.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.670 - 2.200
Rwork	0.230
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1I5Z CRP DIMER
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.075	0.311
Number of reflections	23757
<I/σ(I)>	11.7	3.1
Completeness [%]	96.1	83.4
Redundancy	8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	7.8	298	100MM KCL, 1MM EDTA, 50MM TRIS, 35% GLYCEROL, 20:1 CAMP:CRP, pH 7.8, LIQUID DIFFUSION, temperature 298K
1	LIQUID DIFFUSION	7.8	298	100MM KCL, 1MM EDTA, 50MM TRIS, 35% GLYCEROL, 20:1 CAMP:CRP, pH 7.8, LIQUID DIFFUSION, temperature 298K
1	LIQUID DIFFUSION	7.8	298	100MM KCL, 1MM EDTA, 50MM TRIS, 35% GLYCEROL, 20:1 CAMP:CRP, pH 7.8, LIQUID DIFFUSION, temperature 298K