1I6U

RNA-PROTEIN INTERACTIONS: THE CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN S8/RRNA COMPLEX FROM METHANOCOCCUS JANNASCHII

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-05-17
Detector	MARRESEARCH
Wavelength(s)	0.97624,0.97933,0.97948
Spacegroup name	P 43 21 2
Unit cell lengths	121.687, 121.687, 137.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.070 - 2.600
R-factor	0.218
Rwork	0.218
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.070	2.710
High resolution limit [Å]	2.570	2.570
Rmerge	0.045	0.249
Number of reflections	30271
<I/σ(I)>	13
Completeness [%]	91.3	82.5
Redundancy	7.5	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	4 *	ammonium sulphate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.5	4 *	ammonium sulphate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.5	4 *	ammonium sulphate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	ammonium sulphate
2	1	1	sodium cacodylate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNA	3 (mg/ml)
2	1	drop	protein	3.5 (mg/ml)
3	1	drop	ammonium sulfate	2.8 (M)
4	1	drop	sodium cacodylate	100 (mM)
5	1	reservoir	ammonium sulfate	2.2 (M)
6	1	reservoir	sodium cacodylate	100 (mM)